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ICAR AIEEA (PG) JRF - Plant Biochemistry Syllabus & Exam Pattern

Prepare for your exam with the latest official syllabus, detailed topic-wise breakdown, and mark distribution.

Comprehensive syllabus for the ICAR JRF (Junior Research Fellowship) and SRF (Senior Research Fellowship) entrance exam for the \'Plant Biochemistry\' discipline. Note: This discipline falls under the Major Subject Group \'Plant Biotechnology\' (Code 01). Candidates must prepare for the entire subject group, which includes Plant Biochemistry, Plant Biotechnology & Molecular Biology, and Plant Physiology.

Exam Overview

Conducting Body National Testing Agency (NTA)
Exam Frequency Annual
Major Subject Group Plant Biotechnology (Code 01)
Eligibility B.Sc. Agriculture / Horticulture / Forestry / Sericulture / Biotechnology or B.Sc. (10+2+3 system)
Mode of Exam LAN Based CBT (Computer Based Test)

Exam Pattern

Exam Structure
Section Details Questions Marks
Part A General Knowledge / General Agriculture 20 MCQs 80
Part B Core Subject Group (Plant Biotechnology, Biochemistry, Physiology) 100 MCQs 400
Total 120 MCQs 480

Marking Scheme: +4 for correct answer, -1 for incorrect answer.

Subject-wise Syllabus

Plant Biochemistry (Core)
Molecular Biology
Plant Physiology
General Agriculture
Core Unit: Plant Biochemistry +
  • • Basic Biochemistry & Biomolecules High
    Structure and function of carbohydrates (monosaccharides, oligosaccharides, polysaccharides); Properties of water and pH buffers; Amino acids classification and structure; Primary, secondary, tertiary, and quaternary structure of proteins; Lipids: classification, structure, and functions; Nucleic acids: DNA and RNA structure (A, B, Z forms); Vitamins and Co-enzymes; Biological membranes: structure and transport mechanisms.
  • • Enzymology Very High
    Nomenclature and classification of enzymes; Enzyme kinetics (Michaelis-Menten equation, Lineweaver-Burk plot); Mechanism of enzyme action (lock and key, induced fit); Enzyme inhibition (competitive, non-competitive, uncompetitive); Allosteric enzymes and regulation; Isoenzymes; Ribozymes and Abzymes; Factors affecting enzyme activity (pH, temperature, substrate concentration).
  • • Intermediary Metabolism High
    Glycolysis and its regulation; TCA (Krebs) Cycle; Pentose Phosphate Pathway (HMP shunt); Gluconeogenesis; Glyoxylate cycle; Electron Transport Chain and Oxidative Phosphorylation; Fatty acid oxidation (Beta-oxidation) and biosynthesis; Amino acid metabolism: transamination, deamination, urea cycle; Nucleotide biosynthesis (purine and pyrimidine salvage and de novo pathways).
  • • Photosynthesis & Respiration High
    Photosynthetic pigments and light harvesting complexes; Light reaction (Z-scheme) and photophosphorylation; C3 cycle (Calvin cycle); C4 cycle (Hatch-Slack pathway) and CAM pathway; Photorespiration (C2 cycle) and its significance; Factors affecting photosynthesis; Aerobic vs Anaerobic respiration; Cyanide resistant respiration.
  • • Biochemistry of Food Grains, Fruits & Vegetables Medium
    Biochemical composition of cereals, pulses, and oilseeds; Nutritional quality of proteins (essential amino acids); Anti-nutritional factors in crops; Post-harvest biochemistry: climacteric and non-climacteric fruits; Ethylene role in ripening; Changes during fruit ripening; Biochemistry of seed germination.
  • • Techniques in Biochemistry Medium-High
    Chromatography principles (TLC, HPLC, GC, Ion-exchange, Affinity); Electrophoresis (SDS-PAGE, Agarose gel, 2D-PAGE); Centrifugation techniques; Spectroscopy (UV-Visible, NMR, Mass Spectrometry); Radioisotope techniques (tracers, autoradiography); ELISA and blotting techniques (Southern, Northern, Western); PCR principles.
Core Unit: Molecular Biology & Biotechnology +
  • • Central Dogma High
    DNA Replication in prokaryotes and eukaryotes (enzymes involved, mechanism); Transcription process (RNA polymerase, promoters, transcription factors); Post-transcriptional modifications (capping, tailing, splicing); Translation (genetic code, ribosome structure, initiation, elongation, termination); Post-translational modifications.
  • • Gene Regulation Medium
    Operon concept (Lac operon, Trp operon); Regulation in eukaryotes (enhancers, silencers); Epigenetics (methylation, acetylation); RNA interference (RNAi, siRNA, miRNA); Signal transduction pathways.
  • • Recombinant DNA Technology High
    Restriction endonucleases (types and applications); Cloning vectors (plasmids, cosmids, BACs, YACs, bacteriophages); Gene cloning strategies; Gene transfer methods (Agrobacterium-mediated, gene gun, electroporation); Selection and screening of recombinants; DNA sequencing methods (Sanger, Next-Gen Sequencing).
  • • Plant Biotechnology Applications Medium
    Transgenic crops (Bt cotton, Golden rice, Flavr Savr tomato); Marker Assisted Selection (MAS); Molecular markers (RFLP, RAPD, AFLP, SSR, SNP); Plant Tissue Culture: totipotency, organogenesis, somatic embryogenesis, protoplast fusion, somaclonal variation.
Core Unit: Plant Physiology +
  • • Plant Water Relations & Nutrition Medium
    Water potential and its components; Transpiration and stomatal regulation; Absorption and translocation of water; Essential mineral elements: macro and micronutrients; Deficiency symptoms and toxicity; Nitrogen metabolism: biological nitrogen fixation (symbiotic and asymbiotic), nitrate assimilation.
  • • Growth & Development Medium-High
    Plant Growth Regulators (Auxins, Gibberellins, Cytokinins, Abscisic Acid, Ethylene, Brassinosteroids, Jasmonates); Photoperiodism and Vernalization; Phytochrome and Cryptochrome; Seed dormancy and germination physiology; Stress physiology (Drought, Salinity, Temperature stress).
Part A: General Knowledge +
  • • General Agriculture 20 Questions
    Importance of agriculture in national economy; Major crops of India (Rice, Wheat, Sugarcane, Pulses, Oilseeds); Major soils of India; Agro-climatic zones; Green revolution; Famous agricultural scientists and institutes (ICAR, IARI); Current affairs in agriculture; Government schemes (PM-KISAN, etc.).

Recommended Books

Book NameAuthor/PublisherImportance
Principles of Biochemistry Lehninger (Nelson & Cox) Essential (Bible of Biochemistry)
Biochemistry and Molecular Biology of Plants Buchanan, Gruissem, and Jones High (Standard for Plant Biochem)
Plant Physiology and Development Taiz and Zeiger High (For Physiology concepts)
Objective Plant Physiology and Plant Biochemistry V.U. Patil Practice (For MCQs)
Fundamentals of Plant Biochemistry S.L. Mehta or standard ICAR guide Medium

Frequently Asked Questions

Does the exam cover only Plant Biochemistry? +

No. The exam is for the Major Subject Group 'Plant Biotechnology' (Code 01). The paper will contain questions from Plant Biochemistry, Plant Biotechnology/Molecular Biology, and Plant Physiology. You cannot ignore the other two sections.

What is the weightage of the General Agriculture section? +

Section A contains 20 questions (approx. 16% of total marks) covering General Knowledge and General Agriculture. While the core subject is most important, a good score here provides a rank advantage.

Is there negative marking? +

Yes, there is a deduction of 1 mark for every incorrect answer. Correct answers award 4 marks.

Which ICAR institutes are best for Plant Biochemistry? +

IARI (New Delhi) is the premier institute. Others include NDRI, IVRI, and top State Agricultural Universities (SAUs) like GB Pant, PAU Ludhiana, and TNAU.